VeriFi™ Polymerase - 500 units

Description

A versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required.

VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required.

Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.

Features

• High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
• Increased PCR success rates with complex genomic templates (17.5kb and over)
• High yields under standard and fast PCR conditions (10-30s/kb)
• Efficient and specific amplification from challenging templates including GC and AT-rich sequences
• 100x higher fidelity than Taq DNA polymerase
• Generates blunt-end PCR products

VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates. VeriFi™ Polymerase is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb. The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended. VeriFi™ Polymerase is provided with an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required.

Also available as a convenient 2x ready mix with the option of a red dye for direct gel loading. Also available in a aptamer based hot-start version (VeriFi™ Hot Start Polymerase) for increased primer specificity.