VeriFi™ Mix Red - 500 rxns

Description

A convenient high fidelity 2x mix designed for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates. The inclusion of a red dye enables direct loading and tracking during agarose.

VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required.

Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.

Features:

• High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
• Increased PCR success rates with complex genomic templates (17.5kb and over)
• High yields under standard and fast PCR conditions (10-30s/kb)
• Efficient and specific amplification from challenging templates including GC and AT-rich sequences
• 100x higher fidelity than Taq DNA polymerase
• Generates blunt-end PCR products
• Convenient 2x ready mix with red dye for direct gel loading

VeriFi™ Mix Red contains the engineered and highly processive VeriFi™ Polymerase, developed for fast and versatile high fidelity PCR. The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary mutations significantly improve DNA binding and processivity, resulting in shorter extension times (10-30s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb.

The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.

VeriFi™ Mix Red uses an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required.

Also available in a aptamer based hot-start version (VeriFi™ Hot Start Polymerase) for increased primer specificity.