VeriFi™ Polymerase - sample

Description

Free sample. The price is our standard ice package fee, which only applies if the sample is ordered without paid orders delivered on ice.

Features

• High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
• Increased PCR success rates with complex genomic templates (17.5kb and over)
• High yields under standard and fast PCR conditions (10-30s/kb)
• Efficient and specific amplification from challenging templates including GC and AT-rich sequences
• 100x higher fidelity than Taq DNA polymerase
• Generates blunt-end PCR products

A versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required.

VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates.

VeriFi™ Polymerase is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb. The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase.

The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended. VeriFi™ Polymerase is provided with an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required.

Also available as a convenient 2x ready mix with the option of a red dye for direct gel loading