A convenient high fidelity 2x mix designed for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates
Package size: 500 rxns
Not in stock, delivery 1-2 weeks
A convenient high fidelity 2x mix designed for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates.
VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required.
Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
VeriFi™ Mix contains the engineered and highly processive VeriFi™ Polymerase, developed for fast and versatile high fidelity PCR. The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary mutations significantly improve DNA binding and processivity, resulting in shorter extension times (10-30s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
VeriFi™ Mix uses an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required.
Also available in 500 rxns at lower price per rxns. Also available in a aptamer based hot-start version (VeriFi™ Hot Start Polymerase) for increased primer specificity.