GlowMito - 125 µl

Description

GlowMito provides a non-toxic solution to quickly visualize the entire mitochondrial network in live samples.
It quickly penetrates cells and produces a bright & stable red labeling of mitochondria without inducing cell toxicity or altering mitochondrial functions.
Simply add it to your culture medium, and image repeatedly for up to 3 days without washing!
Soluble in water and compatible with 2-photon microscopy, GlowMito is the solution of choice for thick tissues and in vivo experiments.
GlowMito is retained in the mitochondria of live samples following loss of membrane potential.

Technical Information

Colour: Red/near-infrared
Detection method : Fluorescence, one & two-photon excitation
Peak excitation/emission wavelengths: Ex:542 nm / Em:690 nm
Two-photon absorption peak: 790 nm
Two-photon cross-section: 224 GM
Form : Liquid (resuspended in water)
Sub-cellular localization: mitochondria
For use with: epifluorescence & confocal microscopes, flow cytometers
Fixable: no

Kit contents: 1 vial contains 125 µL of an aqueous solution with a concentration of 1 mM for a final volume of 250 mL of imaging medium

Storage: Store at 4°C for up to 7 months. For long-term storage, aliquot and store at -20°C.

Applications

Experimental outputs: live imaging & flow cytometry

GlowMito produces a bright red labeling of the entire mitochondrial network, including those with reduced potential. It is primarily intended to visualize mitochondria based on their presence rather than assessing their electrochemical properties.
lowMito does not need to be washed out: mitochondrial fluorescence will remain stable for up to 3 days without causing toxicity and can therefore be integrated in your long-term experiments.

GlowMito is perfectly suitable for studying mitochondrial morphology, movement (velocity, localization), tracking, dynamics (fusion/fission), biogenesis (replication & growth), autophagy, etc.

GlowMito can be used in combination with other probes (GFP, potentiometric dyes, calcium signaling probes, etc).

We do not recommend its use for the measure of mitochondrial mass or volume density.

Tested and validated on:
- Human cells: HEK293, HeLa, MCF-7, MDA-MB-231, HMLE, UACC-62, U2-OS, Gli36, HAEC, SH-SY5Y, A-172, A549, patient-derived skeletal muscle cells, primary smooth muscle cells & iPSC-derived cardiomyocytes.
- Mice cells: primary cortical neurons
- Monkey cells: COS-7
- Tissues: hiPSC-derived heart tissues & mice isolated pressurized blood vessels
- Parasitic protists: Trichomonas Vaginalis (hydrogenosome labeling)

GlowMito showed no internalization in yeast cells, Entamoeba histolytica, Giardia intestinalis parasites and seedlings.

GlowMito has been designed by Olivier Siri  and Guy Lenaers