Stencell - Allegro

Description

Stencell are ready-to-use silicon chambers that you can stick and remove for cellular confinement or dynamic assays, such as migration or wound-healing.

Stencell was designed to standardize your experiments and minimize the consumption of reagents. It is very helpful when you are working with super expensive reagents or very rare cell lines, and when you want to test various experimental hypothesis.

Technical information Stencell - Allegro

• Dimensions of the sheet: 220 mm x 16 mm
• Number of units per sheets: 50
• Material of the layer of use: Silicone
• Thickness of the layer of use: 250 μm
• Material of the adhesive layers: PET
• Thickness of the adhesive layers: 112 μm
• Standard volume of use: 20 μL
• Maximal volume of use: 30 μL
• Minimal volume of use: 8 μL
• Dimensions of the barrier: 0.50 mm ± 10 %

Stencell general technical information

Material: PDMS
Kit content: 100 stencils (2 sheets of 50 identical stencils)
Thickness: 250 µm
Sizes & shapes:
- Solo: 1 circular well - Diam. 12 mm
- Quartet: 4 circular wells - Diam. 3 mm
- Nonet: 9 circular wells - Diam. 3 mm
- Presto: 2 oblong wells spaced by 0.25 mm
- Allegro: 2 oblong wells spaced by 0.50 mm

Storage: up to 2 years, at room temperature under their protective sheets.

All Stencell Applications

• Cell migration and wound healing with Stencell - Presto and Stencell - Allegro
  • Interaction dominant/dominated cells
  • Defined regions of Rab5A overexpression
• Parallelized experiments with Stencell - Quartet and Stencell - Nonet
  • Differentiation of stem cells or zebrafish embryonic cells
  • Cell behavior in confined conditions
• Confined experiments with Stencell - Solo
  • Fixed epithelial cells growing confluent

Designed by Vincent Studer , Pierre-Olivier Strale and Aurélien Pasturel. They were hosted by the Cell Organ-izers joint research laboratory (CNRS-Alvéole).

Powered by the Stencil technology published in Advanced Healthcare Materials