Actiflash - 5 ml

Actiflash - 5 ml

# LJU-CIN-5
Package size: 5 ml
Brand: Idylle
Actiflash - 5 ml

DKK 4.604,00

Not in stock, delivery 1-2 weeks

Description

Actiflash is a tamoxifen-like caged-inducer called cyclofen. Upon UV-light illumination, optical-induced uncaging of Actiflash quickly releases proteins fused to the modified estrogen receptor ligand-binding domains ERT2. This allows them to translocate into the nucleus, activate transcription (using Gal4-UAS system) or induce recombination (using the Cre-lox one).
The protein expression can thus be simply controlled in time and space thanks to UV-light.

Actiflash has been successfully used with various cell lines as well as animal models:
● Cell lines: CV1, mammalian epithelial cells (MDCK)
● Zebrafish: 12 hpf – 2 dpf embryos
● Mice

 

Actiflash is provided as a powder, which is stable for years at 2-8°C.
nce solubilized in a DMSO solution (typically at 10 mM), Actiflash can be stored at -20°C for several months (when protected form light).

 

Actiflash has been designed by Ludovic Jullien​, Isabelle Aujard​ and Thomas Le Saux

Originally published in ChemBioChem, additional publicatios: ​Feng, Z., Ducos, B., Scerbo, P., Aujard, I., Jullien, L., & Bensimon, D. (2022)

 

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