LJU-CIN-50
A small steroid ligand that activates engineered proteins upon light induction.
Can be used to precisely control protein activity down to the single cell level in live cell cultures and animals.
Package size: 50 mg
DKK 13.327,00
DKK 4.604,00
Not in stock, delivery 1-2 weeks
Actiflash is a tamoxifen-like caged-inducer called cyclofen. Upon UV-light illumination, optical-induced uncaging of Actiflash quickly releases proteins fused to the modified estrogen receptor ligand-binding domains ERT2. This allows them to translocate into the nucleus, activate transcription (using Gal4-UAS system) or induce recombination (using the Cre-lox one).
The protein expression can thus be simply controlled in time and space thanks to UV-light.
Actiflash has been successfully used with various cell lines as well as animal models:
● Cell lines: CV1, mammalian epithelial cells (MDCK)
● Zebrafish: 12 hpf – 2 dpf embryos
● Mice
Actiflash is provided as a powder, which is stable for years at 2-8°C.
nce solubilized in a DMSO solution (typically at 10 mM), Actiflash can be stored at -20°C for several months (when protected form light).
Actiflash has been designed by Ludovic Jullien, Isabelle Aujard and Thomas Le Saux
Originally published in ChemBioChem, additional publicatios: Feng, Z., Ducos, B., Scerbo, P., Aujard, I., Jullien, L., & Bensimon, D. (2022)