Actiflash is a tamoxifen-like caged-inducer called cyclofen. Upon UV-light illumination, optical-induced uncaging of Actiflash quickly releases proteins fused to the modified estrogen receptor ligand-binding domains ERT2. This allows them to translocate into the nucleus, activate transcription (using Gal4-UAS system) or induce recombination (using the Cre-lox one).
The protein expression can thus be simply controlled in time and space thanks to UV-light.
Actiflash has been successfully used with various cell lines as well as animal models:
● Cell lines: CV1, mammalian epithelial cells (MDCK)
● Zebrafish: 12 hpf – 2 dpf embryos
● Mice
Actiflash is provided as a powder, which is stable for years at 2-8°C.
nce solubilized in a DMSO solution (typically at 10 mM), Actiflash can be stored at -20°C for several months (when protected form light).
Actiflash has been designed by Ludovic Jullien, Isabelle Aujard and Thomas Le Saux
Originally published in ChemBioChem, additional publicatios: Feng, Z., Ducos, B., Scerbo, P., Aujard, I., Jullien, L., & Bensimon, D. (2022)
LJU-CIN-5
A small steroid ligand that activates engineered proteins upon light induction.
Can be used to precisely control protein activity down to the single cell level in live cell cultures and animals.
Package size: 5 ml
DKK 4.604,00
Idylle is a tech transfer platform for research tools in life sciences.
This means that all products are innovative and quite new, although already published.
STU-STE-1WL
A ring-shaped PDMS stencil (diam. 12 mm) to conduct confined experiments.
Can be stuck on a wide variety of supports and subsequently removed..
Package size: 100 stencils (2 sheets of 50)
DKK 1.269,00
STU-STE-4WL
A PDMS stencil containing 4 cell culture areas (diam. 3 mm) allowing to easily parallelize your assays and maximise the condition variations in the same culture dish.
Can be stuck on a wide variety of supports and subsequently removed.
Package size: 100 stencils (2 sheets of 50)
DKK 1.269,00
STU-STE-THN
A PDMS stencil containing 2 adjacent cell culture areas separated by a 0.35 mm gap. Parallel cultures and subsquent removal of the Stencell - Allegro allows to study cell-migration and conduct wound-healing assays.
Can be stuck on a wide variety of supports.
Package size: 100 stencils (2 sheets of 50)
DKK 1.269,00
STU-STE-9WL
A PDMS stencil containing 9 cell culture areas (diam. 3 mm) allowing to parallelize easily your assays and maximise the condition variations in the same culture dish.
Can be stuck on a wide variety of supports and subsequently removed.
out of the clean room!
The no-brainer solution for all your micro-volume experiments, anywhere.
Package size: 100 stencils (2 sheets of 50)
DKK 1.269,00
