SpheroTribe - 25 ml

Description

The SpheroTribe solution is composed of methylcellulose, a biologically inert compound dissolved in basal culture medium without any proteins, lipids or growth factors.
You can dilute it in any culture medium of your choice, and add additional compounds as desired (i.e. serum, antibiotics, differentiation factors, etc).

SpheroTribe provides a simple toolkit to generate consistent and robust 3D cell structures (spheroids, organoids) without restriction of cell type.
Simply dilute the SpheroTribe solution into your culture medium of choice, watch your cells turn into uniformly sized 3D spheroids and collect them for your downstream assays.

Once diluted in your culture medium of choice, our concentrated polymer-based solution increases the medium viscosity favouring cell-cell contacts.
SpheroTribe offers a simple method to generate homogeneous 3D cell structures with increased control over their size and shape, which can be easily handled and washed for downstream experiments.

SpheroTribe is particularly useful to boost aggregation when working with challenging cells, minimize variability between samples and improve the consistency of your migration/invasion assays, immunostaining, drug screening or in vivo implantation experiments.

Kit description

In addition to the SpheroTribe solution, the full kit also includes U-bottom plates and wide-opening tips so you have everything you need to get started with your 3D cell culture experiments.

Content 25 ml kit (960 spheroids):
- 25 ml of 5X methylcellulose solution
- 10 x U-bottom 96-well plates
- 2 x racks of 96 pipette tips (200 µl) with a large opening

Also available as 2,5 ml kit (96 spheroids) TDA-SPK-MINIKIT:
- 2.5 m of 5X methylcellulose solution
- 1 x U-bottom 96-well plate
- 20 pipette tips (200 µl) with a large opening

And: 1 x 25 ml methylcellulose (TDA-SPK-2-25 dkk 3.231)

Storage recommendation for the methylcellulose solution: 4°C for (at least) 10 months.

Applications

So far, SpheroTribe has been successfully used for spheroid/organoid formation with the following cell types:
Patient-derived stem-like glioblastoma cells (GB P3, BL13), human glioblastoma cell lines (U87 & T98G), HeLa, human vaginal mucosal melanoma (HMV-II), human primary colorectal cancer cells, human colon cancer cells (HT-29), human breast cancer cells (MDA-MB 231), human induced pluripotent stem cells, monkey kidney fibroblast-like cell line (COS-7), primary neurons from rat embryos (E18) & murine melanoma cells (B16F10).

Experimental assays
Once spheroids have grown to your desired size, you can use them for any kind of assay according to your regular workflow.
The SpheroTribe solution can be readily washed off, leaving a spheroid available for other tests at any stage of your protocol.

Example of in situ assays you can perform directly on the U-bottom plate supplied:

• Live imaging
• Growth/proliferation studies
• Toxicity studies

Examples of downstream assays that might require transferring spheroids to other vessels:

• Invasion & migration assays
• Immunostaining
• Biochemical assays
• Immune infiltration assays
• In vivo implantation

Original publication: Guyon et al. 2020

Other publications using SpheroTribe spheroids: Guyon et al. 2023 , Guyon et al. 2022