UltraScript™ cDNA Synthesis Kit - 100 rxns

Description

Enhanced cDNA synthesis speed and yield with accurate transcript representation. High quality cDNA synthesis is essential for downstream real-time PCR analysis and successful expression studies. qPCRBIO cDNA Synthesis Kit is an easy-to-use 2 tube system specifically developed to generate cDNA for use in real-time PCR.

The reverse transcriptase, buffer system and optimised blend of random hexamers with anchored oligo(dT) primers provide unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.

Features:

• Unbiased representation of 5’ and 3’ mRNA transcript ends
• Sensitive detection of low copy number transcripts
• High cDNA yields from as little as 4pg total RNA
• Reduced RNase H activity
• Simple 2 tube system
• 5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl₂
• 20x thermostable reverse transcriptase blended with RNase inhibitor

The qPCRBIO cDNA Synthesis Kit uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance cDNA synthesis speed and yield with accurate transcript representation. The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo(dT) allow for unbiased, efficient and sensitive cDNA synthesis.

The modified MMLV reverse transcriptase (RTase) is both thermostable and extremely active. The RTase is not inhibited by ribosomal and transfer RNAs making total RNA an ideal substrate.

The enzyme is blended with RNase inhibitor preventing degradation of RNA by contaminating RNase. 5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2. The relative concentrations of random hexamers and anchored oligo(dT) have been optimised for the generation of cDNA for use in real-time PCR experiments. The kit can be used with 4.0pg to 0.4?g total RNA or oligo(dT) purified mRNA.