UltraScript™ 2.0 cDNA Synthesis Kit - 25 rxns

Description

UltraScript™ 2.0 cDNA Synthesis Kit Separate Oligos is designed for fast and reliable cDNA synthesis from a wide range of RNA sample types. The kit contains all the required components for cDNA synthesis, including separate anchored oligo(dT)₁₈ and random hexamers, to produce high quality cDNA suitable for many different downstream applications. The kit utilizes UltraScript 2.0 Reverse Transcriptase (RTase), a robust and highly thermostable modified MMLV reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation.

Features:

• Highly thermostable reverse transcriptase 55°C to 65°C and above
• Advanced RNase inhibitor
• High cDNA yields from as little as 20pg total RNA
• Accurate reverse transcription of GC-rich and highly structured transcripts
• High sensitivity for challenging templates
• Reduced RNase H activity

The RTase is blended with an advanced RNase inhibitor to prevent degradation of RNA by contaminating RNase. UltraScript 2.0 RTase is not inhibited by ribosomal and transfer RNAs making total RNA an ideal substrate. The kit can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNAThe 5x buffer contains enhancers, dNTPs and MgCl2. The anchored oligo(dT)₁₈ and random hexamers are provided separately. The kit can be used with the oligos provided, or with primers of the user’s design.

Also available as a stand-alone enzyme with buffer and a cDNA synthesis kit with separate oligos. Also available in 100 rxns.