PCRBIO Ultra Polymerase - 1000 units

Description

PCRBIO Ultra Polymerase is a highly robust enzyme, designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors.

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

Features:

• Increased PCR success rates with difficult templates
• Exhibits high tolerance to PCR inhibitors
• Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
• Antibody-mediated hot start for unrivalled detection of low copy number templates
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• 3 fold higher fidelity than Taq

PCRBIO Ultra Polymerase is a highly robust enzyme, designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content (up to 80% GC).

PCRBIO Ultra Polymerase exhibits a high tolerance to PCR inhibitors making it the ideal choice for colony and crude sample PCR. PCR products generated are A-tailed and may be cloned into TA cloning vectors. The enzyme is supplied with an optimised buffer containing dNTPs and MgCl2, and is stable at 25°C for 4 weeks.

Hot Start PCRBIO Ultra Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. Our antibody-mediated hot start formulation inhibits polymerase activity during reaction setup until the initial activation step at 95°C. Inactivation below 65°C prevents the formation of primer dimers and non-specific products, enabling specific and sensitive amplification from low copy number target sequences.

Also available as a convenient 2x ready mix with the option of a red dye for direct gel loading.