PCRBIO Ultra Mix Red - 400 rxns

Description

For the amplification of extremely difficult templates, with the convenience of direct loading onto agarose gels without the need for additional loading buffer.

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

Features:

• Increased PCR success rates with difficult templates
• Exhibits high tolerance to PCR inhibitors
• Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
• Antibody-mediated hot start for unrivalled detection of low copy number templates
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• 3 fold higher fidelity than Taq
• Convenient 2x ready mix with red dye for direct gel loading

PCRBIO Ultra Mix Red has been engineered for the amplification of extremely difficult templates, with the convenience of direct loading onto agarose gels without the need for additional loading buffer. The latest polymerase developments are combined with antibody-mediated hot start technology to deliver outstanding performance for all your PCR applications.

PCRBIO Ultra Mix Red is powered by PCRBIO Ultra Polymerase, a highly robust enzyme designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content.

Our antibody-mediated hot start formulation prevents the formation of primer dimers and non-specific products, allowing for specific and sensitive amplification from low copy number target sequences.

PCRBIO Ultra Mix Red has an error rate of approximately 1 error per 5.0 x 10⁵ nucleotides incorporated. PCR products are A-tailed and may be cloned into TA cloning vectors.