VeriFi™ Hot Start Mix Red - 500 rxns

Description

For PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates, with AptaLock™ hot start technology for highly precise PCR, contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

Features:

• AptaLock™ hot start technology for maximised sensitivity and specificity
• Greater success with long and/or GC or AT-rich templates (17.5kb and over)
• High temperature cycling – up to 100°C denaturation to better separate GC-rich sequences
• 100x higher fidelity than Taq DNA polymerase
• Room temperature setup
• Reaction mix stability for up to 24 hours both before and after PCR run
• Generates blunt-end PCR products
• 2x ready mix with a red dye for direct gel loading

VeriFi™ Hot Start Mix Red contains the highly processive VeriFi™ Hot Start Polymerase, developed for robust and versatile high fidelity PCR. The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary mutations significantly improve DNA binding and processivity, resulting in shorter extension times (30s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb.

PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits both the 3’-5’ exonuclease activity and 5’- 3’ polymerase activity of the enzyme at ambient temperatures. This unique hot start molecule prevents primer dimer formation and nonspecific amplification to maximise the sensitivity and specificity of your PCR. This feature makes the enzyme highly suitable for multiplexing and enables reactions to be set up at room temperature.

The enhanced accuracy of VeriFi™ Hot Start Polymerase results in fidelity that is approximately 100 times higher than Taq DNA polymerase, making it ideal for applications such as cloning, site-directed mutagenesis and sequencing. VeriFi™ Hot Start Mix Red uses an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes regardless of GC content. PCR products generated with this range of products are blunt ended.