IsoFast™ Bst 1-Step Mix - 100 rxns

Description

IsoFast™ Bst 1-Step Mix is designed for reverse transcription of target RNA and subsequent isothermal amplification in a single tube.

The kit utilises IsoFast™ Bst Polymerase for its strong strand displacement activity, enabling DNA synthesis at a constant temperature (65˚C) without the need for thermal cycling. Representing the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) DNA Polymerase, this portion of the protein catalyses the 5’-3’ synthesis of DNA but does not contain the 5’-3’ exonuclease domain (Mead et al 1991).The enzyme is provided in a convenient 2x mix format.

Reverse transcription of target RNA is carried out by the thermostable and extremely active RTase Go, which is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.

Features

• Validated for qualitative detection of SARS-CoV-2 nucleic acid
• Includes strand-displacing IsoFast™ Bst Polymerase in a 2x mix format
• Supplied with RTase Go plus RNase inhibitor
• Reaction is carried out at 65˚C
• Gives rapid and consistent amplification across a wide range of templates
• Includes an advanced buffer system for higher yield under difficult conditions
• Supplied with a fluorescent dye for real-time detection
• 30 minute protocol

Also available in 500 rxns at a lower price per rxn

Ref: Mead DA, McClary JA, Luckey JA, Kostichka AJ, Witney FR, Smith LM. Bst DNA polymerase permits rapid sequence analysis from nanogram amounts of template. Biotechniques. 1991 Jul;11(1):76-8, 80, 82-87.