DKK 8.352,00
Not in stock, delivery 1-2 weels
IsoFast™ Bst 1-Step Mix is designed for reverse transcription of target RNA and subsequent isothermal amplification in a single tube.
The kit utilises IsoFast™ Bst Polymerase for its strong strand displacement activity, enabling DNA synthesis at a constant temperature (65˚C) without the need for thermal cycling. Representing the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) DNA Polymerase, this portion of the protein catalyses the 5’-3’ synthesis of DNA but does not contain the 5’-3’ exonuclease domain (Mead et al 1991). The enzyme is provided in a convenient 2x mix format.
Reverse transcription of target RNA is carried out by the thermostable and extremely active RTase Go, which is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.
Features
Ref: Mead DA, McClary JA, Luckey JA, Kostichka AJ, Witney FR, Smith LM. Bst DNA polymerase permits rapid sequence analysis from nanogram amounts of template. Biotechniques. 1991 Jul;11(1):76-8, 80, 82-87.
PB25.81-03
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
Package size: 600 x 20 ul rxns
DKK 5.080,00
PB25-81-05
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
Package size: 1000 x 20 ul rxns
DKK 7.288,00
PB25.81-50
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
Package size: 10000 x 20 ul rxns
DKK 61.000,00
PB25.82-01
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
Package size: 200 x 20ul rxrs
DKK 1.848,00
PB25.82-03
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
Package size: 600 x 20 ul rxns
DKK 5.080,00
PB25.83-01
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
Package size: 200 x 20ul rxns
DKK 1.848,00
