Everspark 2.0

Description

Discover the long-lasting Everspark blinking buffer series for prolonged blinking in dSTORM microscopy. No more rushing your imaging. Mount your sample, and get stable blinking for weeks. ​

The Everspark buffer series rely on a physical deoxygenation system. Contrary to classical enzyme-based buffers, Everspark buffers maintain long-term blinking of your sample in dSTORM microscopy with optimal quality of image reconstruction for several weeks. Once mounted on your sample, Everspark will maintain blinking phenomenon of common fluorophores in dSTORM microscopy for up to 2 months when using the Everspark buffer and up to 3.5 months when using the Everspark 2.0 buffer. You do not need to replace the buffer in that timeframe.

The Everspark 2.0 allows high resolution dSTORM imaging with dyes ranging from green to far-red emission, while the Everspark buffer works best with yellow to far-red fluorescent dyes.
Up to 2 colours can be imaged sequentially in the Everspark buffer, while 3-colour dSTORM microscopy can be performed in the Everspark 2.0.

The Everspark kit contains ready-to-use Everspark blinking buffers, and the required reagents to seal your sample. All you need to have on your end is your imaging vessel of choice, a coverslip and 1ml syringes. For green fluorophore dSTORM imaging in Everspark 2.0, the 488 nm laser should have sufficient power to get a high quality image in the green channel. We recommend using a laser >200mW, ideally 500mW for optimal results.

Everspark 2

Compatible fluorophores: green, yellow & far-red
Validated fluorophores include AF 488, MemBright 488, FITC, FAM, Spy555, CF 568 & AF 647

Multicolor imaging : +++

Blinking stability (after mounting): up to 3.5 months

Blinking events: +++

Kit description:

• 10 vials with 450 µL of Everspark buffer at 100 mM MEA in Tris.
• 1 vial per experiment.

Vials lifetime: Up to 6 months when stored at 2-8°C in their closed pouch (e.g. unmounted).

Everspark technology has been intially developed by Karine Monier, Arnaud Favier and Christophe Place and published in Scientific Reports: Provost et al. 2019
List of other publications here