CHROMagar™ ESBL - 5000 ml

Description

Chromogenic medium for overnight detecton of gram-negatve bacteria producing extended spectrum beta-lactamase.

The product is composed of a powder base (CHROMagar Orientation) and 1 supplement (CHROMagar ESBL supplement).

MEDIUM PERFORMANCE:
CHROMagar™ ESBL supplement added to CHROMagar™ Orientation contributes to the rapid screening of Gram-negative ESBL-producing bacteria.
CHROMagar™ ESBL supplement allows the detection of ESBL-producing bacteria while inhibiting the growth of other bacteria, including most of those carrying ampC type resistance. This is an important feature because intrinsic ampC resistance has less epidemic relevance, but often leads to ESBL false positive reading in the classical testing methods.
Detection of resistant isolates is difficult based on routine susceptibility testing performed by a clinical microbiology laboratory, while with CHROMagar™ ESBL supplement:
1. Detection after overnight incubation of Gram-negative bacteria expressing a reduced susceptibility to antibiotics of the C3G family.
2. Good species identification thanks to the chromogenic performances of supplemented CHROMagar™ Orientation. Indeed, the product is composed of a powder base CHROMagar™ Orientation and a supplement to enhance ESBL-producing bacteria.
3. High sensitivity (98 %) and Specificity (97 %)*
*"Detection of Extended-spectrum Beta Lactamase producing Enterobacteriaceae" G. Klysova and al. ECCMID 2016. Poster available at the CHROMagar product page
3. Time and workload savings: there is no need of a selective pre-enrichment.
4. Flexibility: CHROMagar™ ESBL supplement is supplied in powder form with a shelf-life of more than 18 months. This allows for flexibility of use, whether in an epidemic situation with many patients to screen, or whether for random surveillance of cultures.

TYPICAL APPEARANCE OF MICROORGANISMS:
E.coli ESBL → Dark pink to reddish
Sensitive Gram negative strains → inhibited
Klebsiella, Enterobacter, Citrobacter → metallic blue
Proteus → brown halo

ESBL (Extended Spectrum β-Lactamases) are enzymes that mediate resistance to penicillins, extended-spectrum third generaton cephalosporins (C3G) and monobactams. ESBL-producing Enterobacteriaceae started to appear in the 80s, and have since emerged as some of the most signifcant hospital-acquired infectons with Escherichia coli and Klebsiella spp. being the main actors, but other gram-negatve species have also been observed. Therefore, the early detecton of ESBL-producing bacteria carriers is important to minimise their impact and the spread of infectons and customise therapeutc patent treatment.

Also available in 25 L (ESRT3-25 = RT413-25 + Supplement ES373-25)