AgarSqueezer - wafer 2.5 µm

Description

AgarSqueezer is a device designed to study cell response to short and long term mechanical confinement within a physiological rigidity range.
The compression height can be choosen among according to the wafer sizes: 2.5 µm, 5 µm,30 µm, 100 µm.

• Material : Silicium Prime type SEMI-standard
• Doped with phosphor
• Resistivity : 1-30 Ω.cm
• Orientation : <100>
• Thickness : 525 μm (+/- 25 μm)

Applications
Agarsqueezer has been successfully used to confine adherent and non-adherent cells, including:

• Human: primary T-lymphocytes, TF1 & ML2 leukemic cells, HS27A fibroblasts, MCF10A breast cells, MDA-MB-231 breast cancer cells, U-2 OS osteosarcoma cells, PC-3 & DU 145 prostate cancer cells, HT29 & HCT116 colorectal adenocarcinoma cells and HT1080 fibrosarcoma cells
• Murine: megakaryocytes, osteocyte-like cells MLO-Y4, primary muscle cells & primary dendritic cells
• Plant: cells from Arabidopsis roots
• 3D cell cultures: mice gastruloïds

Compatible assays

• Live imaging*: cell viability, proliferation, migration, morphology, nuclear deformability, cytoskeleton reorganization, cell tracking, etc
• Standard molecular analysis (immunostaining, western blot, qPCR, flow cytometry, etc): changes in gene/protein expression, proliferation,

Agarsqueezer has been designed by Audrey Prunet, Gilles Simon, Hélène Delanoë-Ayari, Véronique Maguer-Satta and Charlotte Rivière (ILM Lyon, France).

Original publication:Prunet et al. 20 (21). Additional publication: Mouelhi et al. 2023