qPCRBIO SyGreen Mix Fluorescein, 50 ml bottle - 5000 rxns

Description

Can be used to relaibly quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest effeincy.

qPCRBIO SyGreen Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR. For selection of ROX level or noROX you can eventually consult the qPCRBIO selection table: https://pcrbio.com/app/uploads/qPCRBIO-Selection-Table-2020.pdf

qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency.

Features

• Rapid extension rate for early Ct values
• Market-leading sensitivity – increased limit of detection
• Specific amplification from complex templates including GC-rich sequences
• Non-PCR inhibiting intercalating dye
• Also available as an easy-to-see blue mix
• Compatible with all standard and fast cycling real-time instruments

Combined with the latest advancements in polymerase technology and advanced buffer chemistry, qPCRBIO SyGreen Mix offers market leading performance with minimal optimisation. qPCRBIO SyGreen Mix uses a proprietary intercalating dye which does not inhibit PCR, unlike other popular dyes.

qPCRBIO SyGreen Mix uses proprietary antibodymediated hot start technology that prevents the formation of primer-dimers to improve reaction sensitivity and specificity.

High-throughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions. 2x qPCRBIO SyGreen Mix with Fluorescein contains 20nM Fluorescein. This is for instruments which have a well-factor correction feature. Instruments which have this feature are Biorad iCycler®,MyiQ™ and iQ®5 cyclers.