qPCRBIO SyGreen 1-Step Go Hi-ROX 1200 rxns

Description

qPCRBIO SyGreen 1-Step Kits have been designed for fast, highly specific and ultra-sensitive real-time RT-PCR.The kits use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.

Features:

• qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg - 100ng total RNA
• Thermostable reverse transcriptase 45°C to 55°C
• Advanced RNase inhibitor
• Non-PCR inhibiting intercalating dye
• Rapid extension rate for early Ct values
• Market-leading sensitivity
• Increased limit of detection
• Antibody-mediated hot start PCR
• Compatible on all standard and fast cycling real-time PCR platforms

qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template concentrations. The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase.

PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market- leading performance with minimal or no optimisation.For selection of ROX level or noROX you can eventually consult the qPCRBIO selection table: https://pcrbio.com/app/uploads/qPCRBIO-Selection-Table-2020.pdf