qPCRBIO Probe 1-Step Go No-ROX - 50 000 rxns

Description

qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and sensitive probe-based real-time RT-PCR.

The kit is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacon probes.For selection of ROX level or noROX you can eventually consult the qPCRBIO selection table: https://pcrbio.com/app/uploads/qPCRBIO-Selection-Table-2020.pdf

Features

• Rapid and sensitive detection of RNA viruses including SARS-CoV-2
• Rapid extension rate for early Ct values
• Market-leading sensitivity – increased limit of detection
• High efficiency in multiplex reactions
• Advanced RNase inhibitor
• Antibody-mediated hot start PCR
• Compatible with all real-time PCR platforms – standard and fast cycling conditions

The kits use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube. qPCRBIO Probe 1-Step Go is engineered for use on a wide range of probe technologies including TaqMan®, Scorpions® and molecular beacons probes. The kit includes a 2x mix and can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences.

qPCRBIO Probe 1-Step Go is designed to give rapid and accurate results over a broad range of template concentrations and is ideally suited to the detection of RNA viruses including SARS-CoV-2. The kit includes a thermostable and extremely active modified MMLV reverse transcriptase (RTase Go) and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. Antibody-mediated hot start technology prevents the formation of primer dimers and non-specific products giving highly specific and ultra-sensitive real-time RT-PCR with unrivalled efficiency in multiplex. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market-leading performance with minimal or no optimisation.