qPCRBIO HRM Mix - 2000 rxns

Description

For reliable and accurate detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.

qPCRBIO HRM Mix produces ultra-sensitive melt profiles allowing accurate discrimination of subtle sequence variations including class I to class IV mutations and CpG methylation differences.

Features

• Fast and accurate detection of subtle sequence differences
• Ultra-sensitive fluorescence profiles for clear characterisation of samples
• Third generation saturating dye SyGreen 2
• Antibody-mediated hot start technology for improved sensitivity
• Compatible with all real-time instruments with HRM capability

Combined with the latest advancements in polymerase technology and advanced buffer chemistry, qPCRBIO HRM Mix offers market-leading accuracy in High Resolution Melt (HRM) analysis.

qPCRBIO HRM Mix uses SyGreen 2, a 3rd generation, saturating, intercalating dye which does not inhibit PCR.

HRM analysis is a powerful technique for the analysis of mutations, polymorphisms and epigenetic differences in double-stranded DNA samples. HRM analysis can further be used for fast screening of diversity changes, based on melting profile of 16SrRNA gene or other amplicons.https://aem.asm.org/content/aem/80/12/3568.full.pdf

qPCRBIO HRM Mix uses antibody-mediated hot start technology that prevents the formation of primer-dimers to improve reaction sensitivity and specificity. High-throughput screening has resulted in a buffer system that allows efficient amplification from GC-rich and AT-rich templates, under fast and standard cycling conditions.