PCRBIO HS Taq Mix Red - 1000 rxns

Description

PCRBIO HS Taq Mix Red uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity with the added convenience of a pre-loaded red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Whether you need a hot start assay for high-throughput, automated reaction setup or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading enzyme to meet your needs.

Features

• Red mix for direct loading onto agarose gels
• Increased PCR success rates with amplicons up to 6kb
• Ultra low background DNA
• Advanced buffer chemistry including Mg and dNTPs
• Amplifies under standard and fast cycling conditions
• Efficient specific amplification from complex templates including GC rich and AT rich sequences
• Inhibitor tolerant PCR direct from bacterial culture, blood and urine
• Stable at 25°C and 37°C for 4 weeks

Applications
• Genotyping
• High throughput PCR
• Low copy template detection
• Standard and fast PCR
• Multiplex PCR
• TA cloning
• PCR direct from blood
• Colony PCR
• PCR of methylated DNA for bi-sulphite sequencing
• Routine PCR
• “Difficult” PCR - GC/AT rich DNA

PCRBIO HS Taq Mix Red is powered by PCRBIO HS Taq DNA Polymerase to give superior performance on complex templates such as mammalian genomic DNA.

PCRBIO HS Taq Mix Red uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity with the added convenience of a pre-loaded red dye suitable for direct loading and tracking during agarose gel electrophoresis.

“Hot start” is a term used to describe the inactivation of a DNA polymerase until the initial activation step at 95°C. Inactivation below 65°C prevents primer dimer formation and non-specific amplification allowing for specific amplification from low copy number target sequences.

Our proprietary small molecule hot start technology offers improved specificity and sensitivity compared to other methods.

PCRBIO HS Taq Mix Red is room temperature stable for 4 weeks and performs consistently well on a broad range of templates (including both GC and AT rich). PCRBIO HS Taq DNA Polymerase production uses an enhanced 12 step purification strategy which includes physical, chemical and enzymatic removal of host DNA.