IsoFast™ Bst Polymerase with Dye - 8000 units

Description

IsoFast™ Bst Polymerase is a recombinant protein expressed in E. coli and represents the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) DNA Polymerase. This portion of the protein catalyses the 5′-3′ synthesis of DNA and has strand displacement activity but does not contain the 5’-3’ exonuclease domain (Mead et al 1991).

IsoFast™ Bst Polymerase offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.

Features

• Has strand-displacing 5′-3′ polymerase activity
• Lacks 5′-3′ exonuclease activity
• DNA synthesis is performed at a constant temperature
• Operates over a broad temperature range, with an optimum of 65ºC
• Gives rapid and consistent amplification across a wide range of templates
• Supplied with an advanced 2-part buffer system for higher yields under difficult conditions
• 30 minute protocol
• Flexible formats with and without fluorescent dye
• Also available as a 2x mix
• Glycerol-free enzyme

Ref: Mead DA, McClary JA, Luckey JA, Kostichka AJ, Witney FR, Smith LM. Bst DNA polymerase permits rapid sequence analysis from nanogram amounts of template. Biotechniques. 1991 Jul;11(1):76-8, 80, 82-87.