IsoFast™ Bst Polymerase with Dye - 8000 units

IsoFast™ Bst Polymerase with Dye - 8000 units

# PB80.11-08
Package size: 8000 units
Brand: PCRBIO
IsoFast™ Bst Polymerase with Dye - 8000 units

DKK 2.952,00

Not in stock, delivery 1-2 weeks

Description

IsoFast™ Bst Polymerase is a recombinant protein expressed in E. coli and represents the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) DNA Polymerase. This portion of the protein catalyses the 5′-3′ synthesis of DNA and has strand displacement activity but does not contain the 5’-3’ exonuclease domain (Mead et al 1991).

IsoFast™ Bst Polymerase offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.

Features

  • Has strand-displacing 5′-3′ polymerase activity
  • Lacks 5′-3′ exonuclease activity
  • DNA synthesis is performed at a constant temperature
  • Operates over a broad temperature range, with an optimum of 65ºC
  • Gives rapid and consistent amplification across a wide range of templates
  • Supplied with an advanced 2-part buffer system for higher yields under difficult conditions
  • 30 minute protocol
  • Flexible formats with and without fluorescent dye
  • Also available as a 2x mix
  • Glycerol-free enzyme

Ref: Mead DA, McClary JA, Luckey JA, Kostichka AJ, Witney FR, Smith LM. Bst DNA polymerase permits rapid sequence analysis from nanogram amounts of template. Biotechniques. 1991 Jul;11(1):76-8, 80, 82-87.

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