CHROMagar™ O157 - 25 L

Description

Chromogenic medium for the selective isolation and differentiation of E. coli O157 in animal and food samples.

The product is composed of a single powder medium.

MEDIUM PERFORMANCE:
1. Easier detection:E. coli O157 is detected by a characteristic mauve colour after only 18 h of incubation, while most other E. coli are blue.
2. Higher specificity of CHROMagar™ E.coli O157 compared to SMAC: The conventional medium for the detection of E. coli O157 is Sorbitol MacConkey (SMAC) Agar, which has very poor specificity, thus exhibiting an abundance of false positives (Proteus, E. hermanii, etc.). Sorbitol Mac Conkey is also difficult to read because there is a change of colouration in the case of prolonged incubation.
3. High Sensitivity:E. coli O157 → 89 %*
*Sensitivity from scientific study: K.A. Bettelheim, 1998. Reliability of CHROMagar O157 for the detection of enterohaemorrhagic E. coli (EHEC) O157 but not EHEC belonging to other serogroups. J.Appl.Microbiol.85:425-428.

TYPICAL APPEARANCE OF MICROORGANISMS:
E. coli O157 → mauve
Other bacteria → steel blue, colourless or inhibited

The E. coli serotype O157:H7 or its non-motile variant O157:H- is the most common VTEC serotype in relation to public health. Its significance was recognized in 1982, following two outbreaks in the USA. Since then, more than 180 outbreaks have been reported worldwide, with an estimated WHO figure of 70 000 infections per year.

Also available in 5000 ml (EE222).