CHROMagar™ COL-APSE - 5000 ml

Description

Chromogenic medium for detection of Colistin resistant Gram-negative bacteria.

The product is composed of a powder base (B) and a supplement (S).

MEDIUM PERFORMANCE:
CHROMagar™ COL-APSE is a sensitive and specific medium for the growth of Colistin resistant bacterial pathogens. It is particularly useful as a primary isolation medium in the surveillance and recovery of Colistin resistant bacteria from complex human, veterinary and environmental samples especially those with plasmid mediated MCR-1 or novel mechanisms of polymyxin resistance.

1. Colorful differentiation of colonies with acquired colistin resistance. Distinguishing between E. coli, Coliforms, Pseudomonas and Acinetobacter.
2. Impressive limit of detection: 10 CFU/mL
3. particularly useful as a primary isolation medium in the surveillance and recovery of Colistin resistant bacteria from complex human, veterinary and environmental samples especially those with plasmid mediated MCR-1 or novel mechanisms of polymyxin resistance.

TYPICAL APPEARANCE OF MICROORGANISMS:
COL-R E. coli → dark pink to reddish
COL-R Coliforms → metallic blue
COL-R Pseudomonas → translucent, +/- natural pigmentation cream to green
COL-R Acinetobacter → cream
Other Gram negative COL-R → colourless, natural pigmentation
Other Gram negative COL-S → inhibited
Other gram positive bacteria→ inhibited

Polymyxin E (colistin) and B are increasingly used as antimicrobials in the treatment of multi-drug resistant bacterial infections. Polymyxin resistance, although intrinsic in Gram-positive and some Gram-negative species (Proteus, Morganella, Serratia), is now a problem in a number of other pathogens (Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica, Klebsiella pneumoniae).*
Resistance arises due to mutations/insertions in genes involved in LPS biosynthesis (lpx, pmrA/B, mgrB, phoP/Q) and/or the acquisition of phosphoethanolomine transferases (PEtN). Of great concern is the recently described plasmid-encoded PEtN, MCR-1, now found worldwide in a range of animal, human and environmental bacterial isolates.*
*Novel Chromogenic Culture Media (CHROMagar™COL-APSE) for the Isolation and Differentiation of Colistin Resistant Gram-negative Pathogens. ECCMID 2017.