Bst polymerase from PCR Biosystems for accelerated DNA and RNA amplification
The new range of IsoFast™ Bst reagents from PCR Biosystems can be used for a variety of techniques including whole genome amplification, multiple displacement amplification and isothermal amplification. Scientists performing DNA and RNA amplification using IsoFast Bst Polymerase can benefit from consistent results over a wide dynamic range and sensitivities down to 3 copies of target DNA (10fg).
Instead of denaturing DNA by using high temperatures, isothermal amplification takes advantage of DNA polymerases that have high strand displacement capabilities, for example Bst or Phi29 DNA polymerases. These enzymes have the ability to ‘unzip’ DNA as they synthesise complementary strands, removing the need for thermocycler equipment as the reaction is carried out at one temperature. Isothermal methods making use of strand displacing DNA polymerases include multiple displacement amplification, whole genome amplification and loop mediated isothermal amplification or LAMP.
PCR Biosystems’ IsoFast™ Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of 65°C.
Find all IsoFast™ Bst procucts and prices here